Chromatin structure in the cellular slime mold Dictyostelium discoideum.
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Chromatin structure in the cellular slime mold Dictyostelium discoideum.
The structure of Dictyostelium discoideum chromatin has been studied by the following techniques: electron microscopy, staphylococcal nuclease digestion, acrylamide gel electrophoresis, sucrose gradient centrifugation, and melting. The basic unit of chromatin is the nucleosome, which is a particle 98.6 A in diameter. Approximately 50% of the chromatin is protected from nuclease digestion, but t...
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The myxamebae of the cellular slime mold feed upon bacteria and multiply by fission. As soon as the food supply is exhausted fission ceases. They aggregate and form migrating pseudoplasmodia, then fruit (Bonner, 1959). Huffman and Olive (1964) reported that in Dwtyostehum &s~oideum some cell fusions were observed at tile very early aggregation stages, but they observed no fusion at later stages...
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The genetic basis of vegetative incompatibility in the cellular slime mold, Dictyostelium discoideum, is elucidated. Vegetatively compatible haploid strains from parasexual diploids at a frequency of between 10(-6) and 10(-5), whereas "escaped" diploids are formed between vegetatively incompatible strains at a frequency of approximately 10(-8). There is probably only a single vegetative incompa...
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The total lipid content of differentiating Dictyostelium discoideum was 100 to 125 mg per g dry weight; of this, about 95 mg per g was polar lipid (mainly phospholipid) and 25 mg per g was neutral lipid in the early stages. During culmination and maturation of the sorocarp, polar lipid declined to 75 mg per g, while neutral lipid increased to 40 mg per g. Per lOlo cells, polar lipid declined fr...
متن کاملSecretion of Lysosomal enzymes in the cellular slime mold, Dictyostelium discoideum.
A number of typical lysosomal enzymes are very efficiently secreted from amoebae of the cellular slime mold, Dictyostelium discoideum, during growth and early development. Using conditions which employ axenically grown amoebae in suspension in a simple starvation buffer, the lysosomal enzymes are secreted at rates that are at least as rapid as during normal development. Unlike normal growth or ...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.2.705